Biological oceanography; Phytoplankton ecology, cell biology and genetics; Regulation of life cycle strategies; Biotic interactions; Populations dynamics.
המחקר במעבדה שלי מתמקד באקולוגיה ובפיזיולוגיה של פיטופלנקטון ימי. באופן ספציפי, אני מעוניין להבין את מחזורי החיים של פיטופלנקטון ואת יחסי הגומלין בין פיטופלנקטון לוירוסים באוקיינוסים. עבודתי משלבת גם גישות אוקיאנוגרפיות לחקר אוכלוסיות טבעיות בים וגם גישות מעבדתיות לבדיקת התגובות של אורגניזמים פיטופלנקטונים באמצעות שיטות אופטיות ומולקולריות.
2016 ISF Equipment Grant - Integrated optical and biophysical platform to study the diversity, spatiotemporal dynamics, biotic interplay and physiology attributes of marine phytoplankton
2016-2020 ISF Research Grant - Life Cycle Strategies in Marine Phytoplankton: Spatiotemporal Dynamics, Biotic Interactions and Cellular Responses.
Lab overview
I am an experimental biologist generally interested in phytoplankton ecology and evolution as well as marine virology. Phytoplankton comprises a very diverse and heterogeneous assemblage of mainly single-celled microorganisms that form the basis of the marine food web and are major drivers of global biogeochemistry and Earth climate. I find particularly interesting the integrative cross-talk between ecology and cell biology, in order to characterize the dynamics of natural phytoplankton populations at sea, but also assess sub-cellular mechanisms underlying their role in the ecosystem. Over the last years, I’ve been particularly interested on the life cycle on marine phytoplankton, a process recurrently mediated by sexual reproduction (sexual cell fusion and meiosis) and resulting in the differentiation into alternate cell forms, which invariably entails major variations in the ecological dynamics and evolution of a given species. Yet, life cycles have been largely overlooked in phytoplankton research. Giving this premise, my lab focuses on the investigation of the main ecological and cellular differentiation between life cycle phases, assessing the role of each phase and the interplay with other organisms in the natural environment as well as on searching for triggers that regulate life phase transition. I believe that a better knowledge of phytoplankton life cycles will provide a holistic view on the functional significance of phytoplankton species and improve our understanding of the oceans and its responses to environmental changes.
An important phytoplankton group that we study in the lab are the coccolithophores, which constitute an ancient lineage that emerged in the late Triassic (~225 Million years ago) and today comprise over 200 species worldwide distributed. Characteristically, coccolithophore cells are covered to beautifully ornamented scales made of calcium carbonate, named coccoliths. Coccolithophores are of remarkable interest to a wide range of scientists. For marine biologists, they are among the main primary producers, and play a distinct role in oceanic ecosystems. For biogeochemists, they are significant in the global Carbon and Sulphur cycles contributing to the long-term storage of export of organic and inorganic carbon to the deep-ocean and ocean-atmosphere gas exchanges. For paleontologists and geologists, the remarkably continuous coccolithophore fossil record makes them ideal tools for biostratigraphy and paleoceanography studies.
Remarkably, coccolithophores possess complex sexual life cycles is composed to 2 morphologically distinct phases, one haploid and the other diploid. Currently little is known about the significance of such biphasic life cycle. Why coccolithophores have consistently 2 life cycle phases? What is the functional role and impact of each phase in the ecosystem? What are the triggers that mediate life phase transition? How is it regulated at the molecular level? These are some of the main questions we address in the lab…
The figure above, depicts the coccolithophore Pontosphera japonica collected in the Mediterranean sea that is undergoing life cycle phase transition. Such transtions can be detected as the cells carries 2 types of coccoliths, smaller holococcoliths that are typical of haploid cells (top left) and larger heterococcoliths that are typical of diploid cells (top right). (Frada et a. 2009).
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Frada, M.J., Bendif, E-M., Keuter, S., Probert, I. (submitted) The private life of coccolithophores. Perspectives in Phycology.
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Johnson, M.D., Beaudoin, D., Frada, M.J., Brownlee, E., Stoecker, D.K. (2018) High grazing rates on cryptophyte algae in Chesapeake Bay. Frontiers in Marine Science. doi: 10.3389/fmars.2018.00241
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Barak-Gavish, N., Frada, M.J., Lee, P.A., DiTullio, G.R., Ku, C., Malitsky, S., Aharoni, A., Green, S.J., Rotkopf, R., Kartvelishvily, E., Sheyn, U., Schatz, D., Vardi, A. (2018) Bacterial virulence against an oceanic bloom-forming phytoplankter is mediated by algal DMSP. bioRxiv 321398; doi: https://doi.org/10.1101/321398
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Laber, C.P., Hunter, J.E., Carvalho, F., Collins J.R., Hunter, E., Schieler, B., Boss, E., More, K., Frada, M., Thamatrakoln, K., Brown, C.B., Haramaty, L., Ossolinski, J., Fredricks, H., Nissimov, J.I., Vandzura, R., Sheyn, U., Lehahn, Y., Chant, R.J., Martins, A.M., Coolen, M.J.L., Vardi, A., DiTullio, G.R., Van Mooy, B.A.S., and Bidle, K.D. (2018) Coccolithovirus facilitation of carbon export in the North Atlantic. Nature Microbiology Vol. 3 (5): 537-547. doi: 10.1038/s41564-018-0128-4
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Frada M.J., Rosenwasser S., Ben-Dor S., Shemi A., Sabanay H., Vardi A. (2017) Morphological switch to a resistant subpopulation in response to viral infection in the bloom-forming coccolithophore Emiliania huxleyi. PLoS Pathogens 13 (12): e1006775.
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Rosenwasser, S., Frada, M.J., Pilzer, D., Rotkopf, R., Vardi, A. (2017) Unmasking cell-to-cell heterogeneity in susceptibility to viral infection of a bloom forming algae. bioRxiv 186981; doi: https://doi.org/10.1101/186981
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Hunter, J., Frada, M.J., Fredericks, H. F., Vardi, A., Von Mooy, B. (2015) Molecular insights on viral infection and life cycle differentiation in Emiliania huxleyi from targeted and untargeted lipidomics. Frontiers Marine Science. 2:81. doi: 10.3389/fmars.2015.00081
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Frada, M.J., Vardi, A. (2015) Algal viruses hitchhiking on zooplankton across phytoplankton blooms. Communicative & Integrative Biology 8:3, e1029210.
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Frada, M.J., Schatz, D., Farstey, V., Ossolinski, J.E., Sabanay, H., Ben-Dor, S., Vardi, A. (2014) Zooplankton may serve as transmission vectors for viruses infecting phytoplankton blooms in the ocean. Current Biology Vol.24: 2592-2597.
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Lehahn,Y., Koren, I., Schatz, D., Frada, M., Sheyn, U., Boss, E., Efrati, S., Rudich,Y., Trainic, M., Sharoni, S., Laber, C., DiTullio, G.R., Coolen, M.G.L., Martins, A.M., Van Mooy, B. A. S., Bidle, K.D., and Vardi, A. (2014) Decoupling physical from biological processes to assess the impact of viruses on a mesoscale algal bloom. Current Biology Vol. 24: 2041-2046.
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Thamatrakoln, K., Bailleul, B., Brown, C. M., Gorbunov, M., Kustka, A., Frada, M., Joliot, P., Falkowski, P. G. and Bidle, K. D. (2013) Death-specific protein in a marine diatom regulates photosynthetic responses to iron and light availability. Proceedings of the Natural Academy of Sciences of the United States of America Vol. 110: 20123- 20128.
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Guerra, L.T., Levitan, O., Frada, M.J., Sun, J., Falkowski, P.G., Dismukes, G.C. (2013). Regulatory branch points affecting protein and lipid biosynthesis in the diatom Phaeodactylum tricornutum. Biomass & Bioenergy Vol. 59: 306- 315.
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Frada, M.J., Burrows, E.H., Wyman, K., and Falkowski, P. (2013) Quantum requirements for growth and lipid biosynthesis in the marine diatom Phaeodactylum tricornutum (Bacillariophyceae) in nitrogen replete and limited conditions. Journal of Phycology Vol. 49: 381-388.
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Burrows, E.H., Bennette, N.B., Carrieri, D., Dixon, J.L., Brinker, A., Frada, M., Baldassano, S.N., Falkowski, P., Dismukes, G. C. (2012) Dynamics of lipid biosynthesis and redistribution in the marine diatom Phaeodactylum tricornutum under nitrate deprivation. Bioenergy Research Vol. 5: 876-885.
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Frada M.J., Bidle, K.D., Probert, I., de Vargas, C. (2012) In situ survey of life cycle phases of the coccolithophore Emiliania huxleyi (Haptophyta). Environmental Microbiology Vol. 14: 1558-1569.
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Frada M., Young J., Cachão M., Lino S., Martins A., Narciso A., Probert I., de Vargas C., (2010) A guide to extant coccolithophores (Calcihaptophycidae, Haptophyta) using light microscopy. Journal of Nannoplankton Research Vol. 31: 58-112.
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Liu H., Probert I., Huitz J., Claustre H., Edvardsen B., Not F., Frada M., Aris-Brosou S. and de Vargas C. (2009) Extreme diversity in noncalcifying haptophytes explains a major pigment paradox in open oceans. Proceedings of the Natural Academy of Sciences of the United States of America Vol. 106: 12803–12808.
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Frada M., Percopo I., Young J., Zingone A., de Vargas C. and Probert I. (2009) First observations of heterococcolithophore-holococcolithophore life cycle combinations in the family Pontosphaeraceae (Haptophyta). Marine Micropaleontology Vol. 71: 20-27.
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Frada M., Probert I., Allen M.J., Wilson W.H. and de Vargas C. (2008) The “Cheshire Cat” escape strategy of the coccolithophore Emiliania huxleyi in response to viral infection. Proceedings of the Natural Academy of Sciences of the United States of America Vol. 105: 15944–15949.
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Frada M., Not F., Probert I. and de Vargas C. (2006) CaCO3 optical detection with fluorescent in situ hybridization: a new method to identify and quantify calcifying microorganisms from the oceans. Journal of Phycology Vol. 42: 1160-1168.
Education
2005-2009 PhD, University Pierre and Marie Curie (France) - Marine Sciences
1998-2002 B.Sc., University of Azores (Portugal) - Biology
Professional Positions
2011-2015 Postdoctoral Associate, Weizmann Institute of Science (Israel) - Marine Microbiology, Cell Biology.
2009-2011 Postdoctoral Associate, Institute of Marine and Coastal Sciences, Rutgers University (USA) – Marine Microbiology, Biochemistry.
2004-2005 Research assistant, Institute of Marine and Coastal Sciences, Rutgers University (USA) – Marine Ecology.
2002-2003 Research assistant, University of Azores (Portugal) – Microbiology.
Current Members
Yoav Avrahami, M.Sc. Student
2017-today: Mixotrophy in marine phytoplankton
Sabine Keuter, Postdoc
2017-today: Phytoplankton seasonal dynamics
Gil Koplovitz, Technician, Dr.
2016-today:
Past Members
Shai Fainsod, M.Sc. Student
2017-2018: Comparative transcriptome in marine phytoplankton
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